Review

resource source identifier antibodies anti phospho eif2α ser51 cell signaling (Cell Signaling Technology Inc)

Cell Signaling Technology Inc is a verified supplier

Cell Signaling Technology Inc manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Cell Signaling Technology Inc resource source identifier antibodies anti phospho eif2α ser51 cell signaling
Resource Source Identifier Antibodies Anti Phospho Eif2α Ser51 Cell Signaling, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/eif2%CE%B1+antibody/pm41875887-824-2-8
Average 86 stars, based on 1 article reviews

resource source identifier antibodies anti phospho eif2α ser51 cell signaling - by Bioz Stars, 2026-09

86/100 stars

Images

other:

Article Title: Role of endoplasmic reticulum stress in cell apoptosis induced by duck hepatitis A virus type 1 infection
Article Snippet: Antibodies against GRP78, PERK, p-PERK, eIF2α, p-eIF2α, ATF4, CHOP and β-actin were purchased from Cell Signaling Technologies.

Article Title: Basal level of ATF4 promotes T cell readiness for activation-induced proliferation
Article Snippet: eIF2α antibody , Cell Signaling , Cat5324S.

Article Title: In Vivo -Active Soluble Epoxide Hydrolase-Targeting PROTACs with Improved Potency and Stability.
Article Snippet: Antibodies Source Catalog Number Observed MW (kDa) Host Dilution eIF2α Cell Signaling Technology 5324 40 Rabbit 1:1,000 IRE1α Cell Signaling Technology 3294 115 Rabbit 1:1,000 PERK Cell Signaling Technology 3192 140 Rabbit 1:1,000 Phospho-eIF2αS751 Cell Signaling Technology 3398 40 Rabbit 1:1,000 PhosphoIRE1αS724 Abcam ab 48187 115 Rabbit 1:10,000 PhosphoPERKT980 Santa Cruz Biotechnology sc-32577 160 Rabbit 1:1,000 sEH Santa Cruz Biotechnology sc-166961 61 Mouse 1:500 β-actin Santa Cruz Biotechnology sc-47778 44 Mouse 1:20,000 S6 Table S5.

Article Title: SREBF1 mediates immunoparalysis of dendritic cells in sepsis by regulating lipid metabolism and endoplasmic reticulum stress.
Article Snippet: Bax (1:1,000; 2772s), cleaved caspase-3 (1:1,000; 9661), PERK (1:1,000; 3192), p-eIF2α (1:1,000; 3398), eIF2α (1:1,000; 5324), and ATF4 (1:1,000; 11815) were from Cell Signaling Technology (Danvers, MA, US).

Article Title: Calcium signaling from damaged lysosomes induces cytoprotective stress granules.
Article Snippet: Lysosomal damage induces stress granule (SG) formation.. However, the importance of SGs in determining cell fate and the precise mechanisms that mediate SG formation in response to lysosomal damage remain unclear.. Here, we describe a novel calciumdependent pathway controlling SG formation, which promotes cell survival during lysosomal damage.

Staining:

Article Title: Mechanism of the effect of Juan-Tong-Yin on endoplasmic reticulum stress-autophagy in endometriosis rats based on protein kinase R-like endoplasmic reticulum kinase/eukaryotic cell initiation factor 2α pathway.
Article Snippet: Objective: To explore the mechanism of Juan-Tong-Yin (JTY) on endoplasmic reticulum (ER) stress-autophagy in endometriosis (EM) rats through the protein kinase R-like endoplasmic reticulum kinase (PERK)/eukaryotic cell initiation factor 2α (eIF2α) autophagy pathway.. Methods: An EM rat model was established.. A total of 70 Sprague–Dawley (SD) rats were randomly divided into the normal control group, model group, JTY high-, mediumand low-dose groups (25.4, 12.7, and 6.35 g/kg, respectively), progesterone group (0.26 mg/kg), and ER stress group (2-DG, 100 mg/kg).

CRP Assay:

Image Search Results

Inhibition of tRNA charging activates the ISR without inducing RNP granules. ( A ) U-2 OS cells were treated with 0.2% DMSO carrier, 20 µM halofuginone (HF), 250 µM sodium arsenite (As), or 1.25 µM thapsigargin (Tg) for 1 h, and Western blotting for phosphorylated and total eIF2α was done. Total protein ( n = 3 independent replicates) and molecular weights (in kilodaltons) are shown. ( B ) Translation activity was measured using bioorthogonal noncanonical amino acid tagging in cells treated as in A . Methionine or azidohomoalanine (AHA) was added 10 min prior to collection. The ratio of nascent to total protein is shown from n = 3 independent replicates. ( C ) U-2 OS cells stably expressing GFP-G3BP1 (green) were treated as in A and fixed, and nuclei were stained with Hoechst (blue). The percentage of cells with SGs was quantified from n = 3 independent experiments; ≥305 cells were counted in each condition. Scale bars, 10 µm. ( D ) Cells were treated as in C , and immunofluorescence and fluorescence in situ hybridization were done to detect UBAP2L (magenta), PABPC1 (cyan), and poly(A) RNA with oligo(dT) probes (yellow) ( n = 2 independent experiments). ( E ) Cells were treated with 0.2% DMSO, 20 µM HF plus 0.2% DMSO, 250 µM arsenite, or 1.25 µM thapsigargin for 1 h, and the percentage of cells with SGs was quantified ( n = 3 independent experiments); ≥287 cells were counted per treatment. ( F ) Cells were treated, and immunofluorescence microscopy was done as in D to detect the P-body marker DCP1A. Quantification of the percentage of cells with P-bodies (PBs) from n = 3 independent experiments is shown; ≥364 cells were counted per treatment. ( G ) Cells were treated as in C to detect XRN1 (magenta) and EDC4 (yellow) by immunofluorescence microscopy ( n = 2 independent experiments). ( H ) U-2 OS cells were treated as in E , and immunofluorescence microscopy was done to detect DCP1A. The percentage of cells with PBs from n = 3 independent experiments is shown; ≥351 cells were counted per treatment across all replicates. Representative images are shown with the average ± SEM for each experiment, and green, gray, and pink points represent the average of each replicate. Statistical significance was assessed with an ordinary one-way ANOVA followed by Tukey's multiple comparisons test. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005, (****) P < 0.001.

Journal: Genes & Development

Article Title: tRNA synthetase activity is required for stress granule and P-body assembly

doi: 10.1101/gad.353535.125

Figure Lengend Snippet: Inhibition of tRNA charging activates the ISR without inducing RNP granules. ( A ) U-2 OS cells were treated with 0.2% DMSO carrier, 20 µM halofuginone (HF), 250 µM sodium arsenite (As), or 1.25 µM thapsigargin (Tg) for 1 h, and Western blotting for phosphorylated and total eIF2α was done. Total protein ( n = 3 independent replicates) and molecular weights (in kilodaltons) are shown. ( B ) Translation activity was measured using bioorthogonal noncanonical amino acid tagging in cells treated as in A . Methionine or azidohomoalanine (AHA) was added 10 min prior to collection. The ratio of nascent to total protein is shown from n = 3 independent replicates. ( C ) U-2 OS cells stably expressing GFP-G3BP1 (green) were treated as in A and fixed, and nuclei were stained with Hoechst (blue). The percentage of cells with SGs was quantified from n = 3 independent experiments; ≥305 cells were counted in each condition. Scale bars, 10 µm. ( D ) Cells were treated as in C , and immunofluorescence and fluorescence in situ hybridization were done to detect UBAP2L (magenta), PABPC1 (cyan), and poly(A) RNA with oligo(dT) probes (yellow) ( n = 2 independent experiments). ( E ) Cells were treated with 0.2% DMSO, 20 µM HF plus 0.2% DMSO, 250 µM arsenite, or 1.25 µM thapsigargin for 1 h, and the percentage of cells with SGs was quantified ( n = 3 independent experiments); ≥287 cells were counted per treatment. ( F ) Cells were treated, and immunofluorescence microscopy was done as in D to detect the P-body marker DCP1A. Quantification of the percentage of cells with P-bodies (PBs) from n = 3 independent experiments is shown; ≥364 cells were counted per treatment. ( G ) Cells were treated as in C to detect XRN1 (magenta) and EDC4 (yellow) by immunofluorescence microscopy ( n = 2 independent experiments). ( H ) U-2 OS cells were treated as in E , and immunofluorescence microscopy was done to detect DCP1A. The percentage of cells with PBs from n = 3 independent experiments is shown; ≥351 cells were counted per treatment across all replicates. Representative images are shown with the average ± SEM for each experiment, and green, gray, and pink points represent the average of each replicate. Statistical significance was assessed with an ordinary one-way ANOVA followed by Tukey's multiple comparisons test. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005, (****) P < 0.001.

Article Snippet: eIF2α , Santa Cruz Biotechnology , SC-133132 , 1:1000 (Western).

Techniques: Inhibition, Western Blot, Activity Assay, Stable Transfection, Expressing, Staining, Immunofluorescence, Fluorescence, In Situ Hybridization, Microscopy, Marker

Ribosome collisions contribute to ISR activation during halofuginone treatment. ( A ) Cells were treated with 0.2% DMSO, 20 µM halofuginone (HF), 250 µM sodium arsenite (As), or 1.25 µM thapsigargin (Tg) with or without 10 µg/mL puromycin (Puro) for 1 h, and P-eIF2α and total eIF2α levels were determined by Western blotting. Quantification of P-eIF2α:eIF2α is shown at the right , and a representative blot with total protein from n = 3 independent experiments shown at the left . ( B ) Western blotting of total and P-eIF2α was done from wild-type (WT) and ZNF598 knockout (KO) cells treated with 20 µM HF for 0.5, 1, or 4 h. A representative blot with total protein is shown ( top ), and results from n = 3 independent replicates were quantified ( bottom ). ( C ) Western blotting of P-eIF2α and total eIF2α from WT and ZNF598 KO cells treated with 20 µM HF for 1 or 4 h in the absence or presence of 10 µg/mL puromycin added 1 h prior to collection. Representative blot with total protein from n = 3 independent experiments ( top ) and quantification of P-eIF2α:eIF2α ( bottom ) are shown. ( D ) Wild-type and ZNF598 KO cells were treated as in B , and Western blotting for GADD34, CReP (marked by an asterisk), and β-tubulin was done with quantification relative to total protein. Representative blots are shown at the top , and results from n = 3 independent experiments are shown below . Molecular weights (in kilodaltons) are indicated on each blot. Quantification is reported as average ± SEM, with pink, gray, and green points representing each replicate. Statistical significance was determined with ordinary one-way ANOVAs and Tukey's multiple comparisons tests. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005, (****) P < 0.001.

Journal: Genes & Development

Article Title: tRNA synthetase activity is required for stress granule and P-body assembly

doi: 10.1101/gad.353535.125

Figure Lengend Snippet: Ribosome collisions contribute to ISR activation during halofuginone treatment. ( A ) Cells were treated with 0.2% DMSO, 20 µM halofuginone (HF), 250 µM sodium arsenite (As), or 1.25 µM thapsigargin (Tg) with or without 10 µg/mL puromycin (Puro) for 1 h, and P-eIF2α and total eIF2α levels were determined by Western blotting. Quantification of P-eIF2α:eIF2α is shown at the right , and a representative blot with total protein from n = 3 independent experiments shown at the left . ( B ) Western blotting of total and P-eIF2α was done from wild-type (WT) and ZNF598 knockout (KO) cells treated with 20 µM HF for 0.5, 1, or 4 h. A representative blot with total protein is shown ( top ), and results from n = 3 independent replicates were quantified ( bottom ). ( C ) Western blotting of P-eIF2α and total eIF2α from WT and ZNF598 KO cells treated with 20 µM HF for 1 or 4 h in the absence or presence of 10 µg/mL puromycin added 1 h prior to collection. Representative blot with total protein from n = 3 independent experiments ( top ) and quantification of P-eIF2α:eIF2α ( bottom ) are shown. ( D ) Wild-type and ZNF598 KO cells were treated as in B , and Western blotting for GADD34, CReP (marked by an asterisk), and β-tubulin was done with quantification relative to total protein. Representative blots are shown at the top , and results from n = 3 independent experiments are shown below . Molecular weights (in kilodaltons) are indicated on each blot. Quantification is reported as average ± SEM, with pink, gray, and green points representing each replicate. Statistical significance was determined with ordinary one-way ANOVAs and Tukey's multiple comparisons tests. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005, (****) P < 0.001.

Article Snippet: eIF2α , Santa Cruz Biotechnology , SC-133132 , 1:1000 (Western).

Techniques: Activation Assay, Western Blot, Knock-Out

Journal: Genes & Development

Article Title: tRNA synthetase activity is required for stress granule and P-body assembly

doi: 10.1101/gad.353535.125

Figure Lengend Snippet: tRNA synthetase inhibition causes persistent ribosome stalls. ( A ) U-2 OS cells expressing GFP-G3BP1 were treated with 20 µM halofuginone (HF) or 0.2% DMSO carrier. Images were collected every 2 h for 16 h and representative images are shown ( n = 3 independent experiments). ( B ) Cells were treated with DMSO (−) or 20 µM HF for 4 or 16 h, and P-eIF2α and total eIF2α were detected by Western blotting. Representative blots with total protein are shown at the left , and the average ± SEM of n = 3 independent experiments is shown at the right . Molecular weights (in kilodaltons) are shown. ( C ) Cells were treated with 20 µM HF for 1 h (black line), 4 h (purple line), or 16 h (green line), and polysome profiling was performed. A representative profile is shown at the left , and the average ± SEM of the polysome:monosome ratio from n = 3 independent replicates is shown at the right . Pink, gray, and green dots represent results from each replicate. ( D ) Cells were treated with either 0.2% DMSO carrier or 20 µM HF in the presence or absence of 250 µM sodium arsenite (As) or 2 µM rocaglamide A (RocA), and GFP-G3BP1 was imaged every 0.5 h for 3 h. Representative images are shown at the left with the average ± SEM. The percentage of cells with SGs from n = 3 independent experiments is shown at the right ; ≥288 cells were counted per treatment across all replicates. ( E ) Cells were treated with 0.2% DMSO (black line), 2 µM RocA (green line), or 2 µM RocA plus 20 µM HF (purple line) for 3 h followed by polysome profiling. Representative profiles are shown at the left , and the average ± SEM from n = 3 independent experiments is shown at the right , with pink, gray, and green dots representing each replicate. ( F ) Cells were untreated (DMSO carrier) or treated for 24, 4, or 1 h with 20 µM anisomycin (ANI) or 20 µM halofuginone, and Western blotting for p-p38 and p-JNK was done, with the total protein shown below . A representative blot of n = 2 independent experiments is shown. Scale bars, 10 µm. Significance was assessed with ordinary one-way ANOVAs and Tukey's multiple comparisons tests. (*) P < 0.05, (**) P < 0.01, (***) P < 0.005.

Article Snippet: eIF2α , Santa Cruz Biotechnology , SC-133132 , 1:1000 (Western).

Techniques: Inhibition, Expressing, Western Blot

Amino acid deprivation activates the integrated stress response without inducing stress granules. ( A ) U-2 OS cells were placed in media without arginine (−Arg) for 1, 4, or 16 h or in the same media with arginine added back (+Arg) for 4 h, and a representative Western blot for total and P-eIF2α with total protein is shown. ( B ) As in A , with glutamine (Gln)-free media or with glutamine added back (+Gln). ( C ) U-2 OS cells stably expressing GFP-G3BP1 (green) were treated as in A , followed by immunofluorescence for P-body (PB) marker DCP1A (magenta), and nuclei were stained with Hoechst (blue). The percentages of cells with stress granules (SGs) and PBs were quantified from n = 3 independent experiments; ≥410 cells were counted for each condition. ( D ) As in C but with glutamine deprivation; ≥312 cells were counted for each condition. ( E ) Cells were treated with DMSO or 40 nM thapsigargin (Tg) for 1 h or starved of arginine or glutamine for 4 h followed by Western blotting for total or P-eIF2α, with total protein shown below . ( F ) GFP-G3BP1-expressing U-2 OS cells were treated with DMSO control or 40 nM Tg for 1 h, and immunofluorescence was done for DCP1A. The average percentage of cells with SGs or PBs is shown at the right ; ≥440 cells were counted per treatment. Scale bars, 10 µm. Molecular weights (in kilodaltons) are shown on each blot. Images are shown for each experiment ( n = 3 independent replicates), with quantifications reported as average ± SEM and with green, gray, and pink points representing the value for each replicate. Statistical significance was assessed with an ordinary one-way ANOVA followed by Tukey's multiple comparisons test for all but F , where a t -test was used. (*) P < 0.05, (**) P < 0.01, (****) P < 0.001.

Journal: Genes & Development

Article Title: tRNA synthetase activity is required for stress granule and P-body assembly

doi: 10.1101/gad.353535.125

Figure Lengend Snippet: Amino acid deprivation activates the integrated stress response without inducing stress granules. ( A ) U-2 OS cells were placed in media without arginine (−Arg) for 1, 4, or 16 h or in the same media with arginine added back (+Arg) for 4 h, and a representative Western blot for total and P-eIF2α with total protein is shown. ( B ) As in A , with glutamine (Gln)-free media or with glutamine added back (+Gln). ( C ) U-2 OS cells stably expressing GFP-G3BP1 (green) were treated as in A , followed by immunofluorescence for P-body (PB) marker DCP1A (magenta), and nuclei were stained with Hoechst (blue). The percentages of cells with stress granules (SGs) and PBs were quantified from n = 3 independent experiments; ≥410 cells were counted for each condition. ( D ) As in C but with glutamine deprivation; ≥312 cells were counted for each condition. ( E ) Cells were treated with DMSO or 40 nM thapsigargin (Tg) for 1 h or starved of arginine or glutamine for 4 h followed by Western blotting for total or P-eIF2α, with total protein shown below . ( F ) GFP-G3BP1-expressing U-2 OS cells were treated with DMSO control or 40 nM Tg for 1 h, and immunofluorescence was done for DCP1A. The average percentage of cells with SGs or PBs is shown at the right ; ≥440 cells were counted per treatment. Scale bars, 10 µm. Molecular weights (in kilodaltons) are shown on each blot. Images are shown for each experiment ( n = 3 independent replicates), with quantifications reported as average ± SEM and with green, gray, and pink points representing the value for each replicate. Statistical significance was assessed with an ordinary one-way ANOVA followed by Tukey's multiple comparisons test for all but F , where a t -test was used. (*) P < 0.05, (**) P < 0.01, (****) P < 0.001.

Article Snippet: eIF2α , Santa Cruz Biotechnology , SC-133132 , 1:1000 (Western).

Techniques: Western Blot, Stable Transfection, Expressing, Immunofluorescence, Marker, Staining, Control

Model depicting the requirement of tRNA synthetase activity for RNP granule assembly. Upon tRNA synthetase inhibition, ribosomes stall, and eIF2α phosphorylation results from uncharged tRNAs and collided ribosomes. Ribosome-associated quality control (RQC) clears stalled, collided ribosomes but cannot resolve ribosomes that stall in the absence of collisions. The association of stalled ribosomes with mRNAs blocks the assembly of stress granules and P-bodies.

Journal: Genes & Development

Article Title: tRNA synthetase activity is required for stress granule and P-body assembly

doi: 10.1101/gad.353535.125

Figure Lengend Snippet: Model depicting the requirement of tRNA synthetase activity for RNP granule assembly. Upon tRNA synthetase inhibition, ribosomes stall, and eIF2α phosphorylation results from uncharged tRNAs and collided ribosomes. Ribosome-associated quality control (RQC) clears stalled, collided ribosomes but cannot resolve ribosomes that stall in the absence of collisions. The association of stalled ribosomes with mRNAs blocks the assembly of stress granules and P-bodies.

Article Snippet: eIF2α , Santa Cruz Biotechnology , SC-133132 , 1:1000 (Western).

Techniques: Activity Assay, Inhibition, Phospho-proteomics, Control

a Representative IHC images of ADRP staining (scale bars = 60 μm). b Quantitative analysis of ADRP staining in different groups of mice ( n = 8 per group). c ‒ e Representative images and quantitative analysis of Oil Red O staining and Nile Red staining in different groups of mice ( n = 8 per group) (scale bars = 200 μm). c , f Representative TEM micrographs and quantitative analysis of lipid droplet areas in renal tubular epithelial cells (TECs) from different groups of mice ( n = 5 per group) (scale bars = 2 µm). g , h Representative TEM micrographs and quantification of dysmorphic mitochondria in renal TECs from different groups of mice ( n = 5 per group) (scale bars = 2 µm). i – l Representative western blotting images and quantitative analysis of ER stress markers, including XBP-1s, p-EIF2α, EIF2α, and CHOP, in different groups of mice ( n = 6 per group). m ‒ o Representative images and quantitative analysis of Oil Red O-stained and Nile Red-stained areas of TCMK-1 cells subjected to different treatments ( n = 3 independent replicates) (scale bars = 50 µm). p The mitochondrial oxygen consumption rates (OCRs) in different groups of TCMK-1 cells were measured via an extracellular flux analyzer. Representative data ( n = 3 independent replicates) are shown. q , r Quantified OCR parameters are presented as the mean ± SD. Statistical analysis was performed by one-way ANOVA (Tukey’s multiple comparisons test). ns not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Cell Discovery

Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction

doi: 10.1038/s41421-026-00873-w

Figure Lengend Snippet: a Representative IHC images of ADRP staining (scale bars = 60 μm). b Quantitative analysis of ADRP staining in different groups of mice ( n = 8 per group). c ‒ e Representative images and quantitative analysis of Oil Red O staining and Nile Red staining in different groups of mice ( n = 8 per group) (scale bars = 200 μm). c , f Representative TEM micrographs and quantitative analysis of lipid droplet areas in renal tubular epithelial cells (TECs) from different groups of mice ( n = 5 per group) (scale bars = 2 µm). g , h Representative TEM micrographs and quantification of dysmorphic mitochondria in renal TECs from different groups of mice ( n = 5 per group) (scale bars = 2 µm). i – l Representative western blotting images and quantitative analysis of ER stress markers, including XBP-1s, p-EIF2α, EIF2α, and CHOP, in different groups of mice ( n = 6 per group). m ‒ o Representative images and quantitative analysis of Oil Red O-stained and Nile Red-stained areas of TCMK-1 cells subjected to different treatments ( n = 3 independent replicates) (scale bars = 50 µm). p The mitochondrial oxygen consumption rates (OCRs) in different groups of TCMK-1 cells were measured via an extracellular flux analyzer. Representative data ( n = 3 independent replicates) are shown. q , r Quantified OCR parameters are presented as the mean ± SD. Statistical analysis was performed by one-way ANOVA (Tukey’s multiple comparisons test). ns not significant; * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Article Snippet: The membranes were blocked with 5% skim milk and then incubated with primary antibodies against C5aR2 (sc-515734; Santa Cruz Biotechnology), PSS1 (ab157222; Abcam), PSS2 (ARP49961_P050; Aviva Systems Biology Corporation), MFN2 (12186-1-AP; Proteintech), XBP-1s (143F; BioLegend), p-EIF2α (28740-1-AP; Proteintech), EIF2α (11170-1-AP; Proteintech), CHOP (15204-1-AP; Proteintech), COX IV (11242-1-AP; Proteintech), calnexin (10427-2-AP; Proteintech), ERK1/2 (4695; Cell Signaling Technology), p-ERK1/2 (4370; Cell Signaling Technology), HA (ab9110, Abcam), FLAG (ab213519, Abcam), α-tubulin (HRP-80762; Proteintech), and β-actin (Ac028; ABclonal).

Techniques: Staining, Western Blot

a Schematic illustration of the experimental design for establishing a dose gradient of P59 administered via subcutaneous injection in db/db mice. b uACR in different groups of mice ( n = 6 per group). c Representative images of PAS staining of the glomeruli and tubulointerstitium (scale bars = 200 μm). d , e Quantitative analysis of mesangial matrix expansion ( d ) and the tubulointerstitial injury index ( e ) in different groups of mice ( n = 6 per group). f UMAP plot showing thirteen populations of kidney cells in vehicle-treated m/m mice, vehicle-treated db/db mice, and P59-treated db/db mice ( n = 3 per group). Each dot corresponds to a single cell and is colored according to the cell type. g Dot plot showing the expression of genes characteristic of each cell population in the scRNA-seq data. h GO enrichment analysis of DEGs between vehicle-treated db/db mice and P59-treated db/db mice. -Log 10 (adjusted P value) > 1.3 was used as the cutoff. i , j Representative images and quantitative analysis of Oil Red O-stained areas in different groups of mice ( n = 6 per group ) . Scale bars = 100 µm. i , k Representative TEM micrographs and quantitative analysis of LD areas in TECs from different groups of mice ( n = 6 per group) (scale bars = 5 µm). l , m Representative TEM micrographs and quantification of the number of dysmorphic mitochondria in TECs in different groups of mice ( n = 6 per group) (scale bars = 5 µm). n Representative western blotting images and quantitative analysis of ER stress markers (XBP-1s, p-EIF2α, EIF2α, and CHOP) in different groups of mice ( n = 6 per group ) . The data in the graphs are presented as the means ± SD. The data were analyzed by two-sided one-way ANOVA with Tukey’s test. ns not significant; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Journal: Cell Discovery

Article Title: Complement 5a receptor 2 attenuates diabetic kidney disease by promoting mitochondria-associated endoplasmic reticulum membrane formation mediated by PSS-MFN2 interaction

doi: 10.1038/s41421-026-00873-w

Figure Lengend Snippet: a Schematic illustration of the experimental design for establishing a dose gradient of P59 administered via subcutaneous injection in db/db mice. b uACR in different groups of mice ( n = 6 per group). c Representative images of PAS staining of the glomeruli and tubulointerstitium (scale bars = 200 μm). d , e Quantitative analysis of mesangial matrix expansion ( d ) and the tubulointerstitial injury index ( e ) in different groups of mice ( n = 6 per group). f UMAP plot showing thirteen populations of kidney cells in vehicle-treated m/m mice, vehicle-treated db/db mice, and P59-treated db/db mice ( n = 3 per group). Each dot corresponds to a single cell and is colored according to the cell type. g Dot plot showing the expression of genes characteristic of each cell population in the scRNA-seq data. h GO enrichment analysis of DEGs between vehicle-treated db/db mice and P59-treated db/db mice. -Log 10 (adjusted P value) > 1.3 was used as the cutoff. i , j Representative images and quantitative analysis of Oil Red O-stained areas in different groups of mice ( n = 6 per group ) . Scale bars = 100 µm. i , k Representative TEM micrographs and quantitative analysis of LD areas in TECs from different groups of mice ( n = 6 per group) (scale bars = 5 µm). l , m Representative TEM micrographs and quantification of the number of dysmorphic mitochondria in TECs in different groups of mice ( n = 6 per group) (scale bars = 5 µm). n Representative western blotting images and quantitative analysis of ER stress markers (XBP-1s, p-EIF2α, EIF2α, and CHOP) in different groups of mice ( n = 6 per group ) . The data in the graphs are presented as the means ± SD. The data were analyzed by two-sided one-way ANOVA with Tukey’s test. ns not significant; ** P < 0.01; *** P < 0.001; **** P < 0.0001.

Techniques: Injection, Staining, Single Cell, Expressing, Western Blot

Review

resource source identifier antibodies anti phospho eif2α ser51 cell signaling (Cell Signaling Technology Inc)

Structured Review

Images

Related Articles

other:

Staining:

CRP Assay:

Similar Products

Image Search Results